ELISA Flex: Human IL-17A/F (HRP)

ELISA Flex: Human IL‑17A/F (HRP)

3523-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT504
Detection mAbMTF411, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human IL-17A/F ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-17A/F in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human IL-17A/F (HRP)
ApplicationELISA
AnalyteIL-17A/F
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of human IL-17A/F.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range25-4000 pg/ml
Representative standard curve for human IL-17A/F ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

Tutorial, Published July 31, 2023

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Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Featured product, Published February 23, 2022

Broadening our IL‑17 portfolio
Differentiate between human IL-17A, IL17-A/F, and IL-17F with our new monoclonals in ELISA, ELISpot, and FluoroSpot.
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IL-17A/F

Analyte description

Interleukin 17A/F (IL-17A/F) is a heterodimer composed of an IL-17A subunit and an IL-17F subunit. It is a proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain immune cells of the innate immune system. 

Alternative namesInterleukin 17A/F, IL-17A/F, IL17A/F, CANDF6, IL-17F, IL17A, ML-1, ML1, CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17
Cell typeTh17
Gene ID112744, 3605